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Inoculation of germinated seeds

Date: (April 27, 2020)

Project Name: Effect of Plant Growth Promoting Rhizobacteria on quantitative traits of rice varieties and some chemical characteristics of paddy soil

Inoculation of germinated seeds with PGPRs:

The Plant Growth Promoting Rhizobacteria (PGPRs) were obtained from the microbial collection of the Soil and Water Research Institute of Iran. Their population after duplication in Nutrient Broth medium was set at about 108 cfu (colony forming unit) per milliliter. Experimental treatments were performed before seed sowing in the nursery and by inoculation of rice germinated seeds with bacterial suspension. For this purpose, the rice seeds are firstly washed with distilled water and placed in a suitable damp cloth to germinate. Then, according to the amount of rice germinated seeds, a certain amount of bacterial suspension is poured into a suitable cleaning container shaken for one hour to ensure that the seeds are completely impregnated with fertilizer. In combination treatments, non-symbiotic bacterial strains were first mixed in equal proportions and then inoculated. After establishing treatments, the seeds were transferred to the nursery. Before seed sowing in the nursery, due to the need of PGPRs to initiate nitrogen fertilizer activity, a certain amount of starter fertilizer was spread in the rice nursery.